Please check our recent publication in ACS Chemical Neuroscience (IF 4.486) a collaborative effort of BioSIM with IBMC/I3S, the Center for Cooperative Research in Biosciences Biscaia (Spain) and AstraZeneca (Cambridge, UK)

Aβ31–35 Decreases Neprilysin-Mediated Alzheimer’s Amyloid-β Peptide Degradation

José P. Leite, Marta G. Lete, Susan B. Fowler, Ana Gimeno, Juliana F. Rocha, Sérgio F. Sousa, Carl I. Webster, Jesús J. Jiménez-Bar̀bero, and Luís Gales*

ACS Chemical Neuroscience (2021) | DOI: 10.1021/acschemneuro.1c00432

Alzheimer’s disease is associated with the deposition of extracellular senile plaques, made primarily of amyloid-β (Aβ), particularly peptides Aβ1–42 and Aβ1–40. Neprilysin, or neutral endopeptidase (NEP), catalyzes proteolysis of the amyloid peptides (Aβ) and is recognized as one of the major regulators of the levels of these peptides in the brain, preventing Aβ accumulation and plaque formation. Here, we used a combination of techniques to elucidate the mechanism of Aβ binding and cleavage by NEP. Our findings indicate that the Aβ31–X cleavage products remain bound to the neprilysin active site, reducing proteolytic activity. Interestingly, it was already shown that this Aβ31–35sequence is also critical for recognition of Aβ peptides by other targets, such as the serpin-enzyme complex receptor in neuronal cells.

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